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1235 biotinylated lectin malii vector laboratories  (Vector Laboratories)


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    Vector Laboratories 1235 biotinylated lectin malii vector laboratories
    1235 Biotinylated Lectin Malii Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1235+biotinylated+lectin+malii+vector+laboratories/Biotinylated+Vicia+Villosa+Lectin+(VVL%2C+VVA)/pm41569849-759-194-198
    Average 95 stars, based on 162 article reviews
    1235 biotinylated lectin malii vector laboratories - by Bioz Stars, 2026-09
    95/100 stars

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    Recombinant:

    Article Title: Comprehensive identification of the genes involved in the expression of Siglec-15 ligands on osteoclast precursors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Kifunensine Millipore Sigma Cat#K1140 Benzyl-α-GalNAc Calbiochem Cat#200100 Eliglustat Selleckchem Cat#S7852 Apilimod Axon Medchem Cat#Axon1369 α2-3 sialidase NEB Cat#P0743 α2-3,6,8 sialidase NEB Cat#P0720 Recombinant mouse Siglec-15-Fc protein Prepared in house Angata et al. 4 N/A Recombinant His8-sfGFP-mouse LRP1 β-propeller domains 4–5 This paper N/A Critical commercial assays TRAP kit Millipore Sigma Cat#387A TaqMan assay Acp5 Thermo Fisher Scientific Cat#4331182; Assay#Mm00475698_m1 TaqMan assay Lrp1 Thermo Fisher Scientific Cat#4331182; Assay#Mm00464608_m1 TaqMan assay Gapdh Thermo Fisher Scientific Cat#4331182; Assay#Mm99999915_g1 Silencer Select siRNA Lrp1 #1 Thermo Fisher Scientific Cat#4390771; Assay#s69312 Silencer Select siRNA Lrp1 #2 Thermo Fisher Scientific Cat#4390771; Assay#s69313 Expi293F cells Thermo Fisher Scientific Cat#A14527 ExpiFectamine 293 Transfection Kit Thermo Fisher Scientific Cat#A14524 Deposited data NGS raw data This paper NCBI BioProject: PRJNA1248064 Experimental models: Cell lines RAW264.7 ATCC Cat#TIB-71; RRID: CVCL_0493 Oligonucleotides Primers for sgRNA clone amplification, see Table S2 IDT Joung et al. 58 N/A Primers for DNA fragment length analysis, see Table S4 Genomics (Taiwan) N/A Software and algorithms Model-based analysis of genome-wide CRISPR/Cas9 knockout (MAGeCK) v0.5.9.4 Li et al. 30 https://sourceforge.net/projects/mageck/ GraphPad Prism v10.6.0 GraphPad Software https://www.graphpad.com/ Other Biotinylated lectin ConA Vector Laboratories CatB-1005 Biotinylated lectin VVL Vector Laboratories Cat#B-1235 Biotinylated lectin MALII Vector Laboratories Cat#B-1265 Biotinylated lectin SNA Vector Laboratories Cat#B-1305 PE-conjugated streptavidin Jackson ImmunoResearch Cat#016-110-084 Cell Reports 45, 116880, February 24, 2026 19 were prepared as previously described.

    TaqMan Assay:

    Article Title: Comprehensive identification of the genes involved in the expression of Siglec-15 ligands on osteoclast precursors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Kifunensine Millipore Sigma Cat#K1140 Benzyl-α-GalNAc Calbiochem Cat#200100 Eliglustat Selleckchem Cat#S7852 Apilimod Axon Medchem Cat#Axon1369 α2-3 sialidase NEB Cat#P0743 α2-3,6,8 sialidase NEB Cat#P0720 Recombinant mouse Siglec-15-Fc protein Prepared in house Angata et al. 4 N/A Recombinant His8-sfGFP-mouse LRP1 β-propeller domains 4–5 This paper N/A Critical commercial assays TRAP kit Millipore Sigma Cat#387A TaqMan assay Acp5 Thermo Fisher Scientific Cat#4331182; Assay#Mm00475698_m1 TaqMan assay Lrp1 Thermo Fisher Scientific Cat#4331182; Assay#Mm00464608_m1 TaqMan assay Gapdh Thermo Fisher Scientific Cat#4331182; Assay#Mm99999915_g1 Silencer Select siRNA Lrp1 #1 Thermo Fisher Scientific Cat#4390771; Assay#s69312 Silencer Select siRNA Lrp1 #2 Thermo Fisher Scientific Cat#4390771; Assay#s69313 Expi293F cells Thermo Fisher Scientific Cat#A14527 ExpiFectamine 293 Transfection Kit Thermo Fisher Scientific Cat#A14524 Deposited data NGS raw data This paper NCBI BioProject: PRJNA1248064 Experimental models: Cell lines RAW264.7 ATCC Cat#TIB-71; RRID: CVCL_0493 Oligonucleotides Primers for sgRNA clone amplification, see Table S2 IDT Joung et al. 58 N/A Primers for DNA fragment length analysis, see Table S4 Genomics (Taiwan) N/A Software and algorithms Model-based analysis of genome-wide CRISPR/Cas9 knockout (MAGeCK) v0.5.9.4 Li et al. 30 https://sourceforge.net/projects/mageck/ GraphPad Prism v10.6.0 GraphPad Software https://www.graphpad.com/ Other Biotinylated lectin ConA Vector Laboratories CatB-1005 Biotinylated lectin VVL Vector Laboratories Cat#B-1235 Biotinylated lectin MALII Vector Laboratories Cat#B-1265 Biotinylated lectin SNA Vector Laboratories Cat#B-1305 PE-conjugated streptavidin Jackson ImmunoResearch Cat#016-110-084 Cell Reports 45, 116880, February 24, 2026 19 were prepared as previously described.

    Transfection:

    Article Title: Comprehensive identification of the genes involved in the expression of Siglec-15 ligands on osteoclast precursors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Kifunensine Millipore Sigma Cat#K1140 Benzyl-α-GalNAc Calbiochem Cat#200100 Eliglustat Selleckchem Cat#S7852 Apilimod Axon Medchem Cat#Axon1369 α2-3 sialidase NEB Cat#P0743 α2-3,6,8 sialidase NEB Cat#P0720 Recombinant mouse Siglec-15-Fc protein Prepared in house Angata et al. 4 N/A Recombinant His8-sfGFP-mouse LRP1 β-propeller domains 4–5 This paper N/A Critical commercial assays TRAP kit Millipore Sigma Cat#387A TaqMan assay Acp5 Thermo Fisher Scientific Cat#4331182; Assay#Mm00475698_m1 TaqMan assay Lrp1 Thermo Fisher Scientific Cat#4331182; Assay#Mm00464608_m1 TaqMan assay Gapdh Thermo Fisher Scientific Cat#4331182; Assay#Mm99999915_g1 Silencer Select siRNA Lrp1 #1 Thermo Fisher Scientific Cat#4390771; Assay#s69312 Silencer Select siRNA Lrp1 #2 Thermo Fisher Scientific Cat#4390771; Assay#s69313 Expi293F cells Thermo Fisher Scientific Cat#A14527 ExpiFectamine 293 Transfection Kit Thermo Fisher Scientific Cat#A14524 Deposited data NGS raw data This paper NCBI BioProject: PRJNA1248064 Experimental models: Cell lines RAW264.7 ATCC Cat#TIB-71; RRID: CVCL_0493 Oligonucleotides Primers for sgRNA clone amplification, see Table S2 IDT Joung et al. 58 N/A Primers for DNA fragment length analysis, see Table S4 Genomics (Taiwan) N/A Software and algorithms Model-based analysis of genome-wide CRISPR/Cas9 knockout (MAGeCK) v0.5.9.4 Li et al. 30 https://sourceforge.net/projects/mageck/ GraphPad Prism v10.6.0 GraphPad Software https://www.graphpad.com/ Other Biotinylated lectin ConA Vector Laboratories CatB-1005 Biotinylated lectin VVL Vector Laboratories Cat#B-1235 Biotinylated lectin MALII Vector Laboratories Cat#B-1265 Biotinylated lectin SNA Vector Laboratories Cat#B-1305 PE-conjugated streptavidin Jackson ImmunoResearch Cat#016-110-084 Cell Reports 45, 116880, February 24, 2026 19 were prepared as previously described.

    Next-Generation Sequencing:

    Article Title: Comprehensive identification of the genes involved in the expression of Siglec-15 ligands on osteoclast precursors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Kifunensine Millipore Sigma Cat#K1140 Benzyl-α-GalNAc Calbiochem Cat#200100 Eliglustat Selleckchem Cat#S7852 Apilimod Axon Medchem Cat#Axon1369 α2-3 sialidase NEB Cat#P0743 α2-3,6,8 sialidase NEB Cat#P0720 Recombinant mouse Siglec-15-Fc protein Prepared in house Angata et al. 4 N/A Recombinant His8-sfGFP-mouse LRP1 β-propeller domains 4–5 This paper N/A Critical commercial assays TRAP kit Millipore Sigma Cat#387A TaqMan assay Acp5 Thermo Fisher Scientific Cat#4331182; Assay#Mm00475698_m1 TaqMan assay Lrp1 Thermo Fisher Scientific Cat#4331182; Assay#Mm00464608_m1 TaqMan assay Gapdh Thermo Fisher Scientific Cat#4331182; Assay#Mm99999915_g1 Silencer Select siRNA Lrp1 #1 Thermo Fisher Scientific Cat#4390771; Assay#s69312 Silencer Select siRNA Lrp1 #2 Thermo Fisher Scientific Cat#4390771; Assay#s69313 Expi293F cells Thermo Fisher Scientific Cat#A14527 ExpiFectamine 293 Transfection Kit Thermo Fisher Scientific Cat#A14524 Deposited data NGS raw data This paper NCBI BioProject: PRJNA1248064 Experimental models: Cell lines RAW264.7 ATCC Cat#TIB-71; RRID: CVCL_0493 Oligonucleotides Primers for sgRNA clone amplification, see Table S2 IDT Joung et al. 58 N/A Primers for DNA fragment length analysis, see Table S4 Genomics (Taiwan) N/A Software and algorithms Model-based analysis of genome-wide CRISPR/Cas9 knockout (MAGeCK) v0.5.9.4 Li et al. 30 https://sourceforge.net/projects/mageck/ GraphPad Prism v10.6.0 GraphPad Software https://www.graphpad.com/ Other Biotinylated lectin ConA Vector Laboratories CatB-1005 Biotinylated lectin VVL Vector Laboratories Cat#B-1235 Biotinylated lectin MALII Vector Laboratories Cat#B-1265 Biotinylated lectin SNA Vector Laboratories Cat#B-1305 PE-conjugated streptavidin Jackson ImmunoResearch Cat#016-110-084 Cell Reports 45, 116880, February 24, 2026 19 were prepared as previously described.

    Amplification:

    Article Title: Comprehensive identification of the genes involved in the expression of Siglec-15 ligands on osteoclast precursors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Kifunensine Millipore Sigma Cat#K1140 Benzyl-α-GalNAc Calbiochem Cat#200100 Eliglustat Selleckchem Cat#S7852 Apilimod Axon Medchem Cat#Axon1369 α2-3 sialidase NEB Cat#P0743 α2-3,6,8 sialidase NEB Cat#P0720 Recombinant mouse Siglec-15-Fc protein Prepared in house Angata et al. 4 N/A Recombinant His8-sfGFP-mouse LRP1 β-propeller domains 4–5 This paper N/A Critical commercial assays TRAP kit Millipore Sigma Cat#387A TaqMan assay Acp5 Thermo Fisher Scientific Cat#4331182; Assay#Mm00475698_m1 TaqMan assay Lrp1 Thermo Fisher Scientific Cat#4331182; Assay#Mm00464608_m1 TaqMan assay Gapdh Thermo Fisher Scientific Cat#4331182; Assay#Mm99999915_g1 Silencer Select siRNA Lrp1 #1 Thermo Fisher Scientific Cat#4390771; Assay#s69312 Silencer Select siRNA Lrp1 #2 Thermo Fisher Scientific Cat#4390771; Assay#s69313 Expi293F cells Thermo Fisher Scientific Cat#A14527 ExpiFectamine 293 Transfection Kit Thermo Fisher Scientific Cat#A14524 Deposited data NGS raw data This paper NCBI BioProject: PRJNA1248064 Experimental models: Cell lines RAW264.7 ATCC Cat#TIB-71; RRID: CVCL_0493 Oligonucleotides Primers for sgRNA clone amplification, see Table S2 IDT Joung et al. 58 N/A Primers for DNA fragment length analysis, see Table S4 Genomics (Taiwan) N/A Software and algorithms Model-based analysis of genome-wide CRISPR/Cas9 knockout (MAGeCK) v0.5.9.4 Li et al. 30 https://sourceforge.net/projects/mageck/ GraphPad Prism v10.6.0 GraphPad Software https://www.graphpad.com/ Other Biotinylated lectin ConA Vector Laboratories CatB-1005 Biotinylated lectin VVL Vector Laboratories Cat#B-1235 Biotinylated lectin MALII Vector Laboratories Cat#B-1265 Biotinylated lectin SNA Vector Laboratories Cat#B-1305 PE-conjugated streptavidin Jackson ImmunoResearch Cat#016-110-084 Cell Reports 45, 116880, February 24, 2026 19 were prepared as previously described.

    Software:

    Article Title: Comprehensive identification of the genes involved in the expression of Siglec-15 ligands on osteoclast precursors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Kifunensine Millipore Sigma Cat#K1140 Benzyl-α-GalNAc Calbiochem Cat#200100 Eliglustat Selleckchem Cat#S7852 Apilimod Axon Medchem Cat#Axon1369 α2-3 sialidase NEB Cat#P0743 α2-3,6,8 sialidase NEB Cat#P0720 Recombinant mouse Siglec-15-Fc protein Prepared in house Angata et al. 4 N/A Recombinant His8-sfGFP-mouse LRP1 β-propeller domains 4–5 This paper N/A Critical commercial assays TRAP kit Millipore Sigma Cat#387A TaqMan assay Acp5 Thermo Fisher Scientific Cat#4331182; Assay#Mm00475698_m1 TaqMan assay Lrp1 Thermo Fisher Scientific Cat#4331182; Assay#Mm00464608_m1 TaqMan assay Gapdh Thermo Fisher Scientific Cat#4331182; Assay#Mm99999915_g1 Silencer Select siRNA Lrp1 #1 Thermo Fisher Scientific Cat#4390771; Assay#s69312 Silencer Select siRNA Lrp1 #2 Thermo Fisher Scientific Cat#4390771; Assay#s69313 Expi293F cells Thermo Fisher Scientific Cat#A14527 ExpiFectamine 293 Transfection Kit Thermo Fisher Scientific Cat#A14524 Deposited data NGS raw data This paper NCBI BioProject: PRJNA1248064 Experimental models: Cell lines RAW264.7 ATCC Cat#TIB-71; RRID: CVCL_0493 Oligonucleotides Primers for sgRNA clone amplification, see Table S2 IDT Joung et al. 58 N/A Primers for DNA fragment length analysis, see Table S4 Genomics (Taiwan) N/A Software and algorithms Model-based analysis of genome-wide CRISPR/Cas9 knockout (MAGeCK) v0.5.9.4 Li et al. 30 https://sourceforge.net/projects/mageck/ GraphPad Prism v10.6.0 GraphPad Software https://www.graphpad.com/ Other Biotinylated lectin ConA Vector Laboratories CatB-1005 Biotinylated lectin VVL Vector Laboratories Cat#B-1235 Biotinylated lectin MALII Vector Laboratories Cat#B-1265 Biotinylated lectin SNA Vector Laboratories Cat#B-1305 PE-conjugated streptavidin Jackson ImmunoResearch Cat#016-110-084 Cell Reports 45, 116880, February 24, 2026 19 were prepared as previously described.

    Genome Wide:

    Article Title: Comprehensive identification of the genes involved in the expression of Siglec-15 ligands on osteoclast precursors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Kifunensine Millipore Sigma Cat#K1140 Benzyl-α-GalNAc Calbiochem Cat#200100 Eliglustat Selleckchem Cat#S7852 Apilimod Axon Medchem Cat#Axon1369 α2-3 sialidase NEB Cat#P0743 α2-3,6,8 sialidase NEB Cat#P0720 Recombinant mouse Siglec-15-Fc protein Prepared in house Angata et al. 4 N/A Recombinant His8-sfGFP-mouse LRP1 β-propeller domains 4–5 This paper N/A Critical commercial assays TRAP kit Millipore Sigma Cat#387A TaqMan assay Acp5 Thermo Fisher Scientific Cat#4331182; Assay#Mm00475698_m1 TaqMan assay Lrp1 Thermo Fisher Scientific Cat#4331182; Assay#Mm00464608_m1 TaqMan assay Gapdh Thermo Fisher Scientific Cat#4331182; Assay#Mm99999915_g1 Silencer Select siRNA Lrp1 #1 Thermo Fisher Scientific Cat#4390771; Assay#s69312 Silencer Select siRNA Lrp1 #2 Thermo Fisher Scientific Cat#4390771; Assay#s69313 Expi293F cells Thermo Fisher Scientific Cat#A14527 ExpiFectamine 293 Transfection Kit Thermo Fisher Scientific Cat#A14524 Deposited data NGS raw data This paper NCBI BioProject: PRJNA1248064 Experimental models: Cell lines RAW264.7 ATCC Cat#TIB-71; RRID: CVCL_0493 Oligonucleotides Primers for sgRNA clone amplification, see Table S2 IDT Joung et al. 58 N/A Primers for DNA fragment length analysis, see Table S4 Genomics (Taiwan) N/A Software and algorithms Model-based analysis of genome-wide CRISPR/Cas9 knockout (MAGeCK) v0.5.9.4 Li et al. 30 https://sourceforge.net/projects/mageck/ GraphPad Prism v10.6.0 GraphPad Software https://www.graphpad.com/ Other Biotinylated lectin ConA Vector Laboratories CatB-1005 Biotinylated lectin VVL Vector Laboratories Cat#B-1235 Biotinylated lectin MALII Vector Laboratories Cat#B-1265 Biotinylated lectin SNA Vector Laboratories Cat#B-1305 PE-conjugated streptavidin Jackson ImmunoResearch Cat#016-110-084 Cell Reports 45, 116880, February 24, 2026 19 were prepared as previously described.

    CRISPR:

    Article Title: Comprehensive identification of the genes involved in the expression of Siglec-15 ligands on osteoclast precursors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Kifunensine Millipore Sigma Cat#K1140 Benzyl-α-GalNAc Calbiochem Cat#200100 Eliglustat Selleckchem Cat#S7852 Apilimod Axon Medchem Cat#Axon1369 α2-3 sialidase NEB Cat#P0743 α2-3,6,8 sialidase NEB Cat#P0720 Recombinant mouse Siglec-15-Fc protein Prepared in house Angata et al. 4 N/A Recombinant His8-sfGFP-mouse LRP1 β-propeller domains 4–5 This paper N/A Critical commercial assays TRAP kit Millipore Sigma Cat#387A TaqMan assay Acp5 Thermo Fisher Scientific Cat#4331182; Assay#Mm00475698_m1 TaqMan assay Lrp1 Thermo Fisher Scientific Cat#4331182; Assay#Mm00464608_m1 TaqMan assay Gapdh Thermo Fisher Scientific Cat#4331182; Assay#Mm99999915_g1 Silencer Select siRNA Lrp1 #1 Thermo Fisher Scientific Cat#4390771; Assay#s69312 Silencer Select siRNA Lrp1 #2 Thermo Fisher Scientific Cat#4390771; Assay#s69313 Expi293F cells Thermo Fisher Scientific Cat#A14527 ExpiFectamine 293 Transfection Kit Thermo Fisher Scientific Cat#A14524 Deposited data NGS raw data This paper NCBI BioProject: PRJNA1248064 Experimental models: Cell lines RAW264.7 ATCC Cat#TIB-71; RRID: CVCL_0493 Oligonucleotides Primers for sgRNA clone amplification, see Table S2 IDT Joung et al. 58 N/A Primers for DNA fragment length analysis, see Table S4 Genomics (Taiwan) N/A Software and algorithms Model-based analysis of genome-wide CRISPR/Cas9 knockout (MAGeCK) v0.5.9.4 Li et al. 30 https://sourceforge.net/projects/mageck/ GraphPad Prism v10.6.0 GraphPad Software https://www.graphpad.com/ Other Biotinylated lectin ConA Vector Laboratories CatB-1005 Biotinylated lectin VVL Vector Laboratories Cat#B-1235 Biotinylated lectin MALII Vector Laboratories Cat#B-1265 Biotinylated lectin SNA Vector Laboratories Cat#B-1305 PE-conjugated streptavidin Jackson ImmunoResearch Cat#016-110-084 Cell Reports 45, 116880, February 24, 2026 19 were prepared as previously described.

    Knock-Out:

    Article Title: Comprehensive identification of the genes involved in the expression of Siglec-15 ligands on osteoclast precursors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Kifunensine Millipore Sigma Cat#K1140 Benzyl-α-GalNAc Calbiochem Cat#200100 Eliglustat Selleckchem Cat#S7852 Apilimod Axon Medchem Cat#Axon1369 α2-3 sialidase NEB Cat#P0743 α2-3,6,8 sialidase NEB Cat#P0720 Recombinant mouse Siglec-15-Fc protein Prepared in house Angata et al. 4 N/A Recombinant His8-sfGFP-mouse LRP1 β-propeller domains 4–5 This paper N/A Critical commercial assays TRAP kit Millipore Sigma Cat#387A TaqMan assay Acp5 Thermo Fisher Scientific Cat#4331182; Assay#Mm00475698_m1 TaqMan assay Lrp1 Thermo Fisher Scientific Cat#4331182; Assay#Mm00464608_m1 TaqMan assay Gapdh Thermo Fisher Scientific Cat#4331182; Assay#Mm99999915_g1 Silencer Select siRNA Lrp1 #1 Thermo Fisher Scientific Cat#4390771; Assay#s69312 Silencer Select siRNA Lrp1 #2 Thermo Fisher Scientific Cat#4390771; Assay#s69313 Expi293F cells Thermo Fisher Scientific Cat#A14527 ExpiFectamine 293 Transfection Kit Thermo Fisher Scientific Cat#A14524 Deposited data NGS raw data This paper NCBI BioProject: PRJNA1248064 Experimental models: Cell lines RAW264.7 ATCC Cat#TIB-71; RRID: CVCL_0493 Oligonucleotides Primers for sgRNA clone amplification, see Table S2 IDT Joung et al. 58 N/A Primers for DNA fragment length analysis, see Table S4 Genomics (Taiwan) N/A Software and algorithms Model-based analysis of genome-wide CRISPR/Cas9 knockout (MAGeCK) v0.5.9.4 Li et al. 30 https://sourceforge.net/projects/mageck/ GraphPad Prism v10.6.0 GraphPad Software https://www.graphpad.com/ Other Biotinylated lectin ConA Vector Laboratories CatB-1005 Biotinylated lectin VVL Vector Laboratories Cat#B-1235 Biotinylated lectin MALII Vector Laboratories Cat#B-1265 Biotinylated lectin SNA Vector Laboratories Cat#B-1305 PE-conjugated streptavidin Jackson ImmunoResearch Cat#016-110-084 Cell Reports 45, 116880, February 24, 2026 19 were prepared as previously described.



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    Vector Laboratories 1235 biotinylated lectin malii vector laboratories
    1235 Biotinylated Lectin Malii Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1235+biotinylated+lectin+malii+vector+laboratories/Biotinylated+Vicia+Villosa+Lectin+(VVL%2C+VVA)/pm41569849-759-194-198
    Average 95 stars, based on 1 article reviews
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